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KMID : 1134819980270040745
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Journal of the Korean Society of Food Science and Nutrition
1998 Volume.27 No. 4 p.745 ~ p.750
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Antimutagenic and Antigenotoxic Effects of Ligularia fischeri Extracts
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Ham Seung-Shi
Lee Sang-Young
Oh Deog-Hwan
Jung Seung-Won
Kim Sang-Heon
Chung Cha-Kwon
Kang Il-Jun
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Abstract
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The antimutagenic and antigenotoxic effects of ethanol, methanol, water and non-heating ethanol extract of Ligularia fischeri were investigated using Ames test and micronucleus test. Four solvent extracts by themselves did not induce mutagenesis. The four extracts of 200㎍/plate showed approximately 84.7%, 77.1%, 72.5% and 71% inhibitory effect on the mutagenesis induced by N-methyl-N’-nitro-N-nitrosoguanidine(MNNG) and 67.9%, 66.8%, 64.6% and 56% inhibition on the mutagenesis by 4-nitroquinoline-1-oxide(4NQO) against TA100 strain, whereas 70.1%, 60.9%, 61.9% and 52.8% inhibitions were observed on the mutagenesis induced by 3-amino-l,4-dimethyl-5H-pyrido[4,3-b]indol(Trp-P-1) in the presence of 200㎍/plate. TA100 strain was more sensitive than TA98 strain by four kinds of extracts on anti mutagenesis. The effects of Ligularia fischeri extracts on the frequencies of micronucleated polychromatic erythrocytes(MNPCEs) induced by MNNG were investigated in the bone marrow. Ten, 20, 40 and 80㎎ g/㎏ of each extract were administered to animals immediately after injection of MNNG and the exposure time was 36 hours. Inhibitory effects of Ligularia fischeri ethanol extracts were 12%,35.3%,58.8%, and 57%, in the presence of 20, 40, 60 and 80㎎/㎏, respectively whereas methanol extracts showed 15.5%, 32.7%, 50.8%, and 57.9% inhibitory effects, respectively. Both extracts showed enhanced antimutagenic and antigenotoxic effects. These results showed a good correlation between antimutagenic effects in in vitro and in in vivo assay.
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KEYWORD
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Ligularia fischeri, antimutagenic effect, antigenotoxic effect, micronucleus test
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